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by kolinko 1221 days ago
In this case it probably works most reliably with around this number of As, and less/more would decrease reliability, butnitmwould still be functional.

Most of genetics is like that.

1 comments

With more As you risk running into the upper limit on sequence length for the virus shell and with less you run the risk of quicker degradation and not enough expression
The first point being only a concern if you are a virus. If instead you are engineering say, an rna vaccine, you could package your transcript into lipofectamine instead of a coronavirus envelope ;) but point taken, there's always an upper limit.
Also a concern when engineering viruses for research or other treatments such as the AAVs
True. I should have said "if you are using a virus"