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by kevviiinn 1220 days ago
With more As you risk running into the upper limit on sequence length for the virus shell and with less you run the risk of quicker degradation and not enough expression
1 comments

The first point being only a concern if you are a virus. If instead you are engineering say, an rna vaccine, you could package your transcript into lipofectamine instead of a coronavirus envelope ;) but point taken, there's always an upper limit.
Also a concern when engineering viruses for research or other treatments such as the AAVs
True. I should have said "if you are using a virus"