| PCR, the Polymerase Chain Reation. https://en.wikipedia.org/wiki/Polymerase_chain_reaction the emphasis in the wiki article is on DNA, the same basic principles apply with RNA. for example you can start with RNA and use reverse trancriptase to produce a DNA sequence, amplify that sequence to a large copy number by repeatedly replicating it then transcribe the DNA to produce large quantities of RNA. or you can start with the DNA [in large quantities of purity] then create many copies of the RNA by repeatedly transcribing the DNA. The practice of artificial [in vitro] gene synthesis can create arbitrary sequences for input to the process[es]. https://en.wikipedia.org/wiki/Artificial_gene_synthesis liposomes are manufactured via biochemical-mechanical process. https://en.wikipedia.org/wiki/Liposome#Manufacturing |
This is not my understanding. PCR works to produce DNA because the DNA polymerase enzyme creates DNA copies from DNA templates, and those copies become templates themselves, feeding back into the chain reaction.
I'm not aware of similar enzymes capable of making RNA copies from RNA templates (RNA polymerases use DNA templates AFAIK).
I was imagining the RNA vaccines would have used a fully synthetic oligo production method, to get the extreme purity required. But it might be that some sort of PCR-like amplification process is used. Would love to read more if someone has details.