[innomatics]

Thanks for the additional links. I guess you would need temperature stable viral RNApol to do direct 'RNA' amplification. Not sure if that exists or if it's even possible due to RNA instability. As you say not so worthwhile due to high errors.

Producing RNA from DNA amplicons (PCR products) makes sense, I'm curious if that is the source of material from in the vaccines, or if it's artificially synthesized?

Would that be a trade secret?

[rolph]

the exact sequences could be proprietary secrets.

when you have determined a sequence of RNA corresponding to a desired protien you may construct a DNA template with modification to suit your purpose.

the DNA polymerase used for DNA PCR is originally from a high tempurature tolerant organism, [thermophillic bcterium] and it is possible via searching high and low to find an organism that bears high temperature tolerant rdRNApol

https://www.sciencedirect.com/topics/biochemistry-genetics-a...

and BTW the original inventor of PCR [kary mullis]

was awake AF on LSD when he conceived of this technology while sitting on a park bench.

https://en.wikipedia.org/wiki/Kary_Mullis

i believe the original PCR was performed MANUALLY! with glassware and perfectionist tedium.

https://en.wikipedia.org/wiki/Kary_Mullis#Use_of_hallucinoge...

you may find this to be interesting:

https://en.wikipedia.org/wiki/Ion_semiconductor_sequencing

[innomatics]

Thanks for the Kary Mullis story. I performed thousands of PCRs and even made some batches of taq many years ago but never knew about its ideation! I knew some pretty out there biochemists though.