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by rolph 2005 days ago
the exact sequences could be proprietary secrets.

when you have determined a sequence of RNA corresponding to a desired protien you may construct a DNA template with modification to suit your purpose.

the DNA polymerase used for DNA PCR is originally from a high tempurature tolerant organism, [thermophillic bcterium] and it is possible via searching high and low to find an organism that bears high temperature tolerant rdRNApol

https://www.sciencedirect.com/topics/biochemistry-genetics-a...

and BTW the original inventor of PCR [kary mullis]

was awake AF on LSD when he conceived of this technology while sitting on a park bench.

https://en.wikipedia.org/wiki/Kary_Mullis

i believe the original PCR was performed MANUALLY! with glassware and perfectionist tedium.

https://en.wikipedia.org/wiki/Kary_Mullis#Use_of_hallucinoge...

you may find this to be interesting:

https://en.wikipedia.org/wiki/Ion_semiconductor_sequencing

1 comments

Thanks for the Kary Mullis story. I performed thousands of PCRs and even made some batches of taq many years ago but never knew about its ideation! I knew some pretty out there biochemists though.