[benjaminva]

Filming is one really interesting application, the other is imaging of single proteins without the need for crystallisation. I wrote a whole PhD thesis about the idea how to reconstruct the three-dimensional electron density from a lot of extremely noisy single proteins shots. It will make a huge class of proteins experimentally accessible that we couldn't structurally look into before.

[pfdietz]

Hasn't cryoelectron microscopy pretty much taken over for protein structure determination?

[benjaminva]

Indeed and it's a really impressive technology. However, there is the cooling involved and a lot of effort goes into sampling "the real deal" without changes of the structure due to the imaging process. In single molecule shots the idea is to form tiny droplets with the natural protein inside and sample them at room temperature. Naturally sample preparation here comes with its own challenges.

[ImPickleVic]

Do you have links to related content?

[benjaminva]

Here you go: https://www.nature.com/articles/s41467-018-04830-4