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by pfdietz 999 days ago
Hasn't cryoelectron microscopy pretty much taken over for protein structure determination?
1 comments

Indeed and it's a really impressive technology. However, there is the cooling involved and a lot of effort goes into sampling "the real deal" without changes of the structure due to the imaging process. In single molecule shots the idea is to form tiny droplets with the natural protein inside and sample them at room temperature. Naturally sample preparation here comes with its own challenges.