[jonmrodriguez]

Did their naive decompression make the genome longer? If so, it probably didn't fit in the capsid. In our version, we had to provide one of the genes (gene F) in a plasmid in the host cell, in order to reduce the length of the decompressed genome to fit inside the capsid. See this section of our paper:

[The naive] decompression added 909 nucleotides to the wild-type genome. We next addressed practical constraints arising from the length of DNA that can be physically packaged within a øX174 capsid without impacts to reproductive fitness. Previous work has shown that the length of a øX174 genome, when packaged in vitro, must be kept within a few percent of the 5386 nucleotide wild-type length in order to avoid any significant fitness decrease ( Aoyama and Hayashi, 1985). Similar results were shown in vivo ( Russell and Muller, 1984). To reduce the decompressed genome length we removed the first 916 nucleotides of gene F, encoding the coat protein ( Air et al., 1978). We chose gene F because a plasmid containing a restriction fragment encoding wild-type gene F was able to complement two conditional gene F mutations ( Avoort et al., 1983). Additionally, the gene F coding sequence is greater than the total of the combined increases needed to implement the øX174.1 genome design. The truncated gene F version of the decompressed genome was named øX174.1f. To complement øX174.1f when transformed into host cells we designed a medium copy vector expressing gene F under control of a rhamnose-inducible promoter ( Fig. S1).

[dnautics]

ah, ok, had no idea that the capsid was that sensitive to genome length! Thanks! So it really did compress it for a useful purpose.