[waiquoo]

There are a number of ways to handle that problem. You could melt the DNA or denature it in a basic solution. In some nanopore designs, the diameter of the nanopore is so small that when the DNA is pulled through, only a single strand can pass which forces the DNA to unzip and untangle. I believe the Oxford Nanopore approach is to use an enzyme to cleave single nucleotides off of the end of the strand one by one. That way the signal is only coming from a single base, which is helpful in the decoding process.

[geographomics]

The DNA would have to undergo purification beforehand anyway, to extract it from the cell and separate it out from the cellular proteins and RNA. So histone removal would be through modified salt concentrations and protease action.

Also I think the method you are describing is their other sequencing approach - this one, as far as I know, passes an intact strand through each pore and examines the electrical conductivity of overlapping 6 base pair sequences.