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by timthimmaiah 4349 days ago
I've done a lot of work on this @ https://www.teselagen.com/

More specifically, we are able to automate combinatorial gene assembly straight from a web app interfacing with a TECAN microfluidic liquid handling robot.

Here's a proof of concept with a simulator, but I've tested this in a live enviornment.

https://www.youtube.com/watch?v=FWA1O0DHEh8

1 comments

Teselagen is cool design software but there's a whole other side of the equation.

I don't see any microfluidics in that video, unless one of those reservoir blocks or plates is actually something else?

You know that's pretty far from really automating combinatorial gene assembly :p You need a lot more than a Tecan to do that kind of cloning; even just the assembly reaction (Gibson?), which is the simplest part is going to be better off in a thermocycler or incubator compared to a warmed deck block. Tecans are pretty unusable at volumes below 2-3 ul, too, so setting up the reaction isn't always straight forward, especially if you need to e.g., PCR fragments out for the assembly.

Transcriptic's API covers the rest of the devices: https://www.transcriptic.com/platform