[lcrs]

You can absolutely tell a difference! Probably more so with synthesized sounds, which don't have natural pitch deviations or complex harmonics. The beating you hear in any even-tempered dyad other than an octave is really obvious with sine or square waves, presumably especially so with the sustained notes in a chorale :)

[dekhn]

There's a blues guy Willie McBride who has recorded blues in just intonation. It's kind of interesting to listen to, not for everybody, but if you're OK with indian and other non-western-classic music, it's worth checking out.