[AaronBBrown]

All decent musicians adjust every note on every instrument (where possible) in order to be in tune with themselves and the others they are performing with. Musicians that don't do this quickly find themselves out of a job (or making millions as the next big pop act). Mostly, this is not done particularly consciously unless you find yourself performing with a group that is either far more accomplished or far less accomplished than yourself. Of course, there are exceptions, namely open strings and piano. However, even in the case of conventional stringed instruments, it is common to adjust fingerings to avoid open strings in order to maintain tone color and more easily adjust intonation.

Basically, while studying temperament is interesting, the reality is it rarely causes practical issues. Most pianos are tuned with equal temperament, which is good enough and almost every other western instrument has enough flexibility to adjust "on the fly."