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by maxharris 4835 days ago
Well, you could try ordering from Fischer or any of the usual research supply places out there, but they will not ship to a non-institutional address.

Even theft isn't an option here, unless you're somehow certain that a particular lab has exactly the right antibody. (In my lab, we often have trouble keeping track of that for ourselves...)

And don't forget that you need to be able to maintain sterile conditions, and have whatever other reagents you need to dilute things, etc.

1 comments

Wait - are you saying people could (were it legal) simply order this stuff from a company? Can you give me some kind of reference for this? If so, I could see people reaching out and finding others capable of placing the order.
Here's CD47, conjugated to a fluorophor (when you have a cell covered in a conjugated antibody, it fluoresces when exposed to laser light - this is done so that an instrument called a flow cytometer can identify cells).

http://www.biolegend.com/percp-cy55-anti-human-cd47-4338.htm...

Of course, to get CD47 depletion, you'd probably want an antibody that isn't conjugated (functional grade):

http://www.ebioscience.com/resources/in-vivo-use-of-antibodi...

Also, some more help on the terminology: CD47 (and most, if not all molecules that fall under the Cluster of Differention [CD] nomenclature) vary on a per-species basis. So an antibody that targets CD47 in mice is not going to stick to human CD47. "Anti-" in this context means that the antibody in question will stick to specific molecule in the species that comes after the "anti-".

Note what it says on the bottom of the page. That's there because of regulations.

Perfect, thanks for all this.