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by hoppp
20 days ago
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The author is working on an opensource C++ codebase and not on biology tasks. The work is around tooling. It's like saying well a scalpel is used for medical reasons, sure. But manufacturing scalpels is metalworking, not medicine. |
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> salmon is a wicked-fast program for highly-accurate, transcript-level quantification from RNA-seq data. It pairs a fast mapping stage — selective alignment, or alignment-free sketch mode (--sketch) — with a massively-parallel statistical model (EM/VBEM over equivalence classes) to estimate transcript abundances. You can give salmon raw sequencing reads, or regular alignments to the transcriptome (an unsorted BAM), and it uses the same inference engine either way.