[amluto]

LNPs have limited capacity, and if the CRISPR/Cas system they’re using requires an exact match, then any additional point mutation or insertion or deletion in the segment being matched would allow the cell to survive. I suppose that approximately 3n extra guide RNAs (where n is the length of the matched sequence) could be added, but that seems a bit messy.

[MontyCarloHall]

Including additional guides is negligible with respect to the overall LNP capacity, which can contain a few thousand nucleotides. The CRISPR/Cas mRNA is about 5 kilobases total, and each guide is on the order of 100 bases.