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by bonsai_spool 57 days ago
> When practitioners say "PCR" they don't (usually) just mean amplifying DNA for use as part of the input to another process.

I definitely do.

> What they usually mean is PCR with chemistry that selectively amplifies some specific sequence of DNA. This chemistry has dyes in it which fluoresce when illuminated at some specific wavelengt

This is called qPCR (and qRT-PCR, and RT-PCR and ‘Taqman assay’... But it's not called PCR because it's not just PCR).. It has uses outside of diagnostics (which is what it seems you're most familiar with).

Either way, the article is not about qPCR.