[nextos]

This is very impressive, top labs doing research often don't have experimental designs that are this elaborate. Was the TCR and BCR-seq you conducted helpful to design cell therapies, neoantigen vaccines, and monitor progress?

Given that you carry the HLA-B*27:05 allele, you might have been blessed by being predisposed to a better response. But probably you want to keep an eye on future autoimmunity issues. Talking from experience...

[sytse]

Thanks for the warning, I hope that it wasn't a personal experience for you.

Thanks for the compliment about the elaborate design. I think that when you make something for one or a few patients it is easier to be more elaborate, even with the same knowledge and equipment.

Maybe the TCR and BCR-seq was most helpful for mRNA design and effectiveness monitoring, but hopefully someone else on my team will answer that better.

[jacobjstern]

The TCR sequencing has been helpful for downselecting TCRs for a TCR based cell therapy, and for monitoring response to various immune therapies (including the vaccines)

[nextos]

Interesting, thanks for your replies.

You should consider publishing a patient case report somewhere, as I believe there are lots of valuable conclusions to be extracted from your work.