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by biophysboy
136 days ago
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It amplifies sequences that contain the two primer sequences on each end of the target. So if you had synthesized sequence XYZ with some mistakes like YZX, then you could target X and Z and purify. You're correct that PCR has a limited max length, but it is longer and cheaper than vanilla DNA synthesis. |
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The Polymerase Chain Reaction
https://www.nobelprize.org/prizes/chemistry/1993/mullis/lect...