Hacker News new | ask | show | jobs
by bonsai_spool 245 days ago
There wasn’t enough information to tell (on my 1 minute scan) which nanopore kit was used, but the presence of PCR does not imply short reads.

You can do nanopore PCR/cDNA workflows right up to the largest known mRNAs (13kb).

Edit:

I’m not sure if you’re saying that you can’t do a 5/20/30X genome on nanopore - that’s also not true. It only makes sense in particular research settings, of course.
1 comments
zorgster 242 days ago
PCR is central to short read sequencing... as well as Roche 454, and can also be used in some protocols in Nanopore.

https://pmc.ncbi.nlm.nih.gov/articles/PMC3849550/

You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion

link
bonsai_spool 239 days ago
> PCR [...] can also be used in some protocols in Nanopore.

Yes... and that is why I said that the presence of PCR does not imply short reads.

> You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion

THat's why I said that 5/20/30X coverage is possible in the appropriate research setting

link