[westurner]

> Here we evaluated diverse corrective CRISPR-based editing approaches that target the ΔGT mutation in NCF1, with the goal of evaluating post-editing genomic outcomes. These approaches include Cas9 ribonucleoprotein (RNP) delivery with single-stranded oligodeoxynucleotide (ssODN) repair templates [11,12], dead Cas9 (dCas9) shielding of NCF113, multiplex single-strand nicks using Cas9 nickase (nCas9) [14,15], or staggered double-strand breaks (DSBs) using Cas12a16.

- "A NICER approach to genome editing with less mutations than CRISPR" (2023) [cas13d] https://news.ycombinator.com/item?id=37698196 :

"Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning" (2023) https://www.nature.com/articles/s41587-023-01830-8