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by pazimzadeh
666 days ago
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If all you're interested is cloning/expression, then I guess that makes sense, although I've not heard anyone complain that 20 min is a slow doubling time. can't you just inoculate your starting culture with a larger amount or something? I often find myself starting cultures late in the day so that they don't overgrow the next day (although I am not doing expression work). Vibrio natriegens seems great for your purposes. I'm curious if there is a tradeoff to its super fast growth. Does it have a greater mutation rate? |
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No, I'm doing high throughput cloning in such a way where a limiting factor is going from 1 cell to X cells (usually pickable colonies). So liquid culture doesn't matter quite as much, since I can sequence validate from a colony split (half into colony PCR, half into new culture), and sequencing takes approximately as much time as the growth step takes.
> I'm curious if there is a tradeoff to its super fast growth. Does it have a greater mutation rate?
Eh, not really. The biggest tradeoff is that it isn't studied nearly as much as E.coli, so the chemical competence protocols kind of suck. I can't actually use it in my pipeline right now because of that. So I'm stuck with E.coli until I can figure out a good transformation protocol.