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by dekhn 897 days ago
Oh. I'm actually looking at the pipette tip under a microscope (in my case it's clear glass that I pulled to a fine tip using heat) and I can see the tardigrade get sucked into the tip when I gently pull with my mouth. And then I can see it whoosh back out when I push. The actual volume doesn't matter, just that I pick a single tardigrade and place it where it needs to go.

The other folks pipetting by mouth are actually looking down at the tube and see the fluid volume reaches a particular well-defined line on the glass pipette. If you can taste the fluid, you've gone too far.