It's technically possible to reuse it, but letsencrypt / certbot do not reuse it by default. You have to go out of your way and do extra work to reuse a CSR when renewing a cert.
The original poster didn't mention LE or anything else that uses ACME. It's pretty easy to reuse a key in a bespoke PKI setup; the X.509 builder APIs that I've used make it trivial. Which doesn't make it a good idea, of course.