[zozbot234]

It's being used to this day (or until very recently, at least) in some countries, generally running under dosbox because DOS software can no longer run natively in modern PC OS's.

[Sunspark]

FreeDOS exists.. https://freedos.org/

[livrem]

That can no longer run on many modern computers as the backwards-compatible BIOS is phased out, not to mention many computers switching to ARM.

[janc_]

You can still run it on qemu (even on ARM).