[theamk]

No, once written to USB stick, ISO image is not a file anymore, it is a sequence of blocks outside of any filesystem. This means you need to use special raw disk tools (like dd) to modify it, and you can no longer operate on it using file managers, "ls", "mv", "rm" and all the related stuff.

You are correct that in black hat scenario, one could rewrite ISO image on the fly - after all, that's what I do when I upgrade my bootable USB stick to latest version.

But while ISOs are not perfect protection against malware, they are _great_ against accidental modification. On my PC, when I insert USB stick, the window pops up right away, and it takes 1 (one) mouse drag to make drive unbootable. No such problem exists with ISO images.