[t_serpico]

How have I missed the point? The answer that nature cannot engineer and can't start de novo are trivially true statements that provide no actual insight into the question. I fully agree the original question itself is a deep one. A quick literature search is more productive than pontificating with weak analogies. See https://www.math.unl.edu/~bdeng1/Papers/DengDNAreplication.p... for what seems to be an interesting analysis regarding base number and DNA replication rate.

[usrbinbash]

> that provide no actual insight into the question

Mind elaborating on that?

Because there is no biochemical reason why DNA could not have incorporated, say, a third pairing pair, so while base-3 (which I don't specifically mention in my post btw.) wouldn't work, base 6 or 8 would have been possible. "Unnatural Base Pairs" are even known to work in laboratory settings.

There is also no biochemical reason why base2 life wouldn't work. Expand the reading frame of the translation machinery to 5 instead of three, and you have enough coding space for polypeptides.

My answer adresses the question completely, because the only reason behind these "decisions" is an ancient system that simply got "frozen", and now cannot change any more.

[sterlind]

> There is also no biochemical reason why base2 life wouldn't work.

are you sure about that? are you sure there's no weird effects that might destabilize very long sequences of 2-nucleotide DNA? or on how wide DNA-binding domains have to be to cope with reduced information density, and how that might sterically hinder smaller arrangements of proteins?

> My answer adresses the question completely, because the only reason behind these "decisions" is an ancient system that simply got "frozen", and now cannot change any more.

your answer is just a hypothesis, not a proof. these things can be studied (by studying abiogenesis in-vitro), and it's not certain these decisions were "flash frozen" like you describe. 2-, 4-, and 6- nucleotide coding systems might have coexisted in the RNA world, and 4- could have won out for some reason.

[usrbinbash]

> are you sure about that?

Yes, I am sure about that, because I used to study Biology before going into IT. And we had a lovely lecture in which we used to discuss theoretical setups for lifeforms at a molecular level.

2 nucleotide DNA isn't necessarily less stable. AT-rich domains have less bindings, but if stablity is the issue, use CG instead (3 bindings)...although that is also a compromise, because then opening DNA for transcription gets more difficult.

> your answer is just a hypothesis, not a proof.

My answer is what we observe in evolutionary biology.

I have given an example outside of the molecular world for a reason. There is no real advantage to the inversion of the neural architecture in Chordata, it just didn't matter when the neural tube formation mechanisms came to be. Now, with mammals having huge brains and complex sensory organs, the warts in that design show.

The proof for that is easy to come by, (also a reason btw. why the neural inversion is my favorite example for this): Look an any Protostomia. Their neural system isn't inverted. Consequently, Squids don't have a visual blind spot.

[sterlind]

your example of the blind spot is quite elegant and convincing. I think it's partly so convincing because there's a large fossil record and diverse phylogenetic tree, with many gaps covered. conversely, we're missing direct evidence for the pre-LUCA era, and what we have is bottlenecked. this makes me more skeptical.

for instance, I've seen arguments that the codon mapping, and even the particular set of protein- coding amino acids, that we ended up with was arbitrary, but I've also read papers arguing that the amino acids include a sort of spanning set of different structural scaffolds with different polarity that happen to mesh well with DNA, and that the particular choices of codons were influenced by how the RNA t-acyl transferases arose, etc.

so, I'm still unconvinced, but I find this area fascinating to read about.

[j33zusjuice]

Idk enough about this discussion to argue it, but his hypothesis does not imply your second point couldn't be true.

> your answer is just a hypothesis, not a proof. these things can be studied (by studying abiogenesis in-vitro), and it's not certain these decisions were "flash frozen" like you describe. 2-, 4-, and 6- nucleotide coding systems might have coexisted in the RNA world, and 4- could have won out for some reason.

His hypothesis is, at least in part, “4- won for some reasons for which we have no explanation, and it stayed that way for some reason [that we may or may not know].” I suppose the reason would be that 4- was somehow better suited for the particular use-case at the time.

Of course there’s a ton of interesting details to discuss to discover, and whether if multiple systems coexisted is one of many fascinating things to discuss, and his response never said otherwise.