[BenFranklin100]

You’re not even responding to the original questions, which is is lightsheet microscopy capable of imaging entire living brains? Pay attention.

[kevviiinn]

Can you use light microscopy on a living brain? No

[BenFranklin100]

Two and three photon microscopy works on living brains down to depth of approximately 1.5 mm. A company even sells head mounted miniature microscopes for mice. Try to refrain from commenting on topics outside your expertise.