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by tripletao 1334 days ago
> As far as I've been able to read, those sites aren't in the final assembly in Shi's papers - just in the primers.

Where did you read that? Kinney explicitly asserts the opposite, but I can't figure out what he's referring to. Someone else linked to Figure S9 from their 2017 PLOS Pathogens paper, which indeed seems not to contain the site in the final assembly. I've edited my other comment here to reflect that.
1 comments
mikeyouse 1333 days ago
Nope - the restriction sites aren't in the final assembly in that paper. These authors just don't understand the tools remotely well enough to have written their paper. It will never be published.

https://twitter.com/alchemytoday/status/1583361758903013376

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tripletao 1333 days ago
Yeah, I think Kinney is wrong here. Someone else found a survey paper noting the theoretical possibility of using BsaI and BsmBI in a way that leaves the sites in the final genome, but I haven't seen anyone who actually did that. So I'm leaning to a false discovery here.
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