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by tripletao 1334 days ago
Their description of the assembly strategy as "Golden Gate" seems like incorrect terminology. The WIV has at least published papers using BsaI and BsmBI, though.

https://twitter.com/jbkinney/status/1583267221047869441

https://twitter.com/jbkinney/status/1583248052969562112

https://journals.plos.org/plospathogens/article?id=10.1371/j...

EDIT: Kinney also asserts here that they left the sites in a final assembled genome (i.e., the genome of a replication-competent virus). I'm still trying to figure out if that's true, though.