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by galangalalgol 1349 days ago
The study dissolved it in DMSO, then that in polysorbate 80, then that in polyethylene glycol. Then injected that intraperitoneally. That seems doable.
3 comments

Intraperitoneal injection isn't really done in humans, the closest equivalent would be subcutaneous or maybe intramuscular injection. I don't know about the safety of injecting DMSO into humans but it's probably not too bad since it seems perfectly safe when used for transdermal drug delivery. The bigger problem is that you're injecting a water-insoluble compound mixed in a small amount of solvent into what is essentially a big bag of water. Once that DMSO/PEG/whatever is injected, it will just mix with the water in your body, so the ISRIB will just precipitate out of solution since it is now surrounded by 99% water molecules. So it will never get distributed to all your organs, which is the goal.
I could be missing something, but since your cells are separated by water, it doesn't matter how many steps you take to get it in your body.
DMSO is soluble in both polar and non polar solvents. I assume the insolubility in water is precisely what they used it to solve. Im any case, their delivery mechanism worked in mice, so it would work in people. The part that might not carry over to people is the effect once in the cell.
I am cringing so hard imaging someone injecting something intraperitoneally. Good luck missing your intestines.