[someweirdperson]

Does the tuning of one string affect the others on guitars with hard tails, or is this mostly a tremolo induced issue?

[pravus]

Yes, for hard-tails. Neck construction doesn't matter either. I notice it on both bolt-on and neck-through instruments. My method is to tune the highest strings first and work down to the lowest since that seems to minimize this effect.

[themarkn]

I believe the issue is due to small changes in the overall tension on the instrument caused by changing one string’s tension.