[alias_neo]

Stand on the right on escalators, let people off of the carriages before you barge on, don't stop suddenly without checking who's about to walk into you, don't run into tube trains as the doors are closing only to get stuck and hold up the train, DON'T HOLD THE DOORS OPEN, go up and down stairs on the left, corridors etc, stay on the left and don't fuck around at the barriers. Have your card ready, your contactless ready, take your kids, cases, whatever through the wide gate. Don't play whatever shitty video you're watching to the whole train and certainly don't subject others to your music taste.

DO ask people for directions or for help, people may look tired, they may look grumpy they may even be those things, but most will be more than willing to help you.

EDIT: Others covered a few important ones I missed too; backpacks off, walking N-abreast so as to be blocking others from passing are a big no-no; you may be having a leisurely stroll, commuters have places to be, whether at work or at home with their families and their feet up after a long day.

[NoboruWataya]

Another one that really gets me - when you get on a half-crowded train, MOVE IN, don't just park yourself by the door blocking everyone behind you.

Of course, these are all rules that should be followed by everyone on all public transport. Nothing really specific to the tube.

[drcongo]

Please run for Mayor of London.

edit: Rule to add - don't just stand still when you step off an escalator.

[wst_]

Adding to this: Don't jump aboard wearing your dirty working jumpsuit smeared with paint, cement, oil. Also stop loudly arguing with your partner over phone. Get your shoes off the chairs.

[dustedcodes]

That's a great summary!