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by dekhn 1367 days ago
No. You can't really adjust your microscope hardware to get a larger depth of field, but field of view is straightforward. You want FOV because most things you are going to look at are relatively thin planes. If you want more DoF you should do photo stacking. Even for FOV, I do multiple field acquisition and then stitch because even my low-mag microscope only covers a 1mm circle.