This is because historically aggressiveness is a male attribute, so when it is present in women it stands out and the perception is amplified. It is the same when a woman is as tall as a man, for example.
It does seem like things are getting better, but certainly when I was growing up almost any behavior the slightest bit feminine in a male was called out as “gay”. The phrases “act like a man” or “real man” are often not just valorizing masculine traits but admonishing feminine ones. So, I’d say very yes.
Yes - you can look at the long history of feminine men getting bullied, no matter their age. Or the decision for eyeliner-wearing men to not wear it to work. (My spouse carried a polka-dot bag that I bought for a while: He, a man in his 40's, found that coworkers laughed. For a while afterwards, he carried it out of spite).