[orena]

Turboreg is also good: Paper: http://bigwww.epfl.ch/publications/thevenaz9801.html

Python implementation: https://pypi.org/project/pystackreg/

[amelius]

Would this work with images which are out of focus?

[orena]

Probably yes, it works on 2p Neuro images, which are of course not InFocus