Source for this? Searching for "Hardest working in show business" without quotes turns up something relating to an entertainer, and searching it with quotes also returns a bunch of entertainment-related matches.
I'm a native speaker that grew up and lives in the U.S. People use it all the time. I don't have any scientific research papers available to show you, but I promise you - this is a common expression.
Misunderstandings about water-transport timetables in 3...2...
* i'll add that whare i am 'the hardest working folks in show business', is often a compliment: we have our backstage (offices etc), and front of house (dealing with customers) where we have our smiley faces plastered on.