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by 100ideas
1867 days ago
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In my experience it’s easier to measure and thus control the temperature of a thermal mass that is much much larger than both the sensors (thermocouples) attached to it and the samples inside of it. The large thermal mass of the heating block in a traditional thermocycler vs the relatively tiny masses of the samples inside the pcr tubes contained within means that the samples are basically always at the temperature of the block, which means the control loop for the heating/cooling system can be modeled and controlled with just a PID feedback loop. |
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