If by major cryptocurrency you mean "only bitcoin and ethereum", then sure. Cardano is the third-largest cryptocurrency by market cap (currently; it's neck-and-neck with a couple more, so this is subject to change still), and has used PoS from day 1 (though I've read some people consider it not "true" PoS because of how they solve the issues of ownership consolidation, and it's also been called "delegated PoS")