[bmsran]

One minor correction - in step 2 the DNA is not amplified as this would reduce the fragment length and also lose the methylation information

[murphyslab]

Good point. Here they're starting from a cell line, so presumably just starting with as much DNA as they can get from the cells. Amplification is usually needed in other scenarios where the sample is more finite, though from what I've read, nanopore sequencing tech doesn't need much DNA.