[mrfusion]

Great write up! Thanks!

Regarding step 1 how can any human have an entire homozygous X chromosome?

Also/rather why not just use a male with one X chromosome?

[bmsran]

there are long regions on the Y chromosome that are very similar to the X chromosome, which would make the analysis difficult:

https://en.wikipedia.org/wiki/Pseudoautosomal_region