[twic]

> Since we know what both the reference and viral signals look like, we can perform linear regression analysis to fit the linear combination of viral and reference signals that best match our data.

Does this mean you assume a linear relationship between the quantity of viral RNA and the strength of the signal?

I know that when back i used to draw calibration curves from my positive controls, there was usually a sublinear relationship, across all sorts of different assays, at least at the upper end.