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by pmiller2 2295 days ago
Yeah, I don't understand why it's literally illegal to take a swab and run a PCR against the known, published viral sequence. Forget "any self-respecting wetlab biologist," I'm pretty sure an undergrad with a year's worth of lab experience could run the test, at least under supervision.

Edit: I suppose you do need a "self respecting wetlab biologist" to synthesize the primers, but running the test itself is pretty simple.

1 comments

Running the test is simple but getting good primers nowadays is easy because they can be bought and arrive 24 hours later. If the sequence is published, then even the lab manager can get them.
Yes, labs don't synthesize their own primers. They buy them from suppliers. The kind of primers you would use for SARs-Cov2 would be about $10-$20AUD (similar for USD) a vial and be sufficient for several hundred reactions (maybe a hundred or so tests with triplicate replicates, pos and negative controls). The thermocycler and the technician doing the pipetting are the expensive bits, not the primers!