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by searine 3064 days ago
>The error rate is stupidly high (somewhere between 10 and 20%)

The Insertion/deletion error rate is 20-30%.

The point mutation error rate is something 0.1-1% (higher than HiSeq but not crazy high).

This means with a semi-decent reference genome you should be able to do re-sequencing fairly accurately. It also means, that in conjunction with HiSeq reads you can do cheap genome assembly, using the HiSeq reads for coverage, and the minion reads for scaffolding.

1 comments

Do you have a citation for this? Because this is not my understanding.
I can add to this with my anecdotal evidence. In my experience (looked at ~15gb of basecalled data total), there is a large amount of indel errors.

The mismatch rate is much lower. But it's hard to calculate exactly the mismatch rate when the indel rate is so high.

No citation, just personal experience aligning the data and comparing it directly to a hiSeq run of the same DNA.

I was able to get about 1-10% mutation rate, with a median of about 1.5%. Rate depending on quality of the run. In general it was on par with PacBio.