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by mee_too
3300 days ago
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But if CRISPR cuts off a single DNA sequence, surely the repair mechanisms won't damage a random gene far away. My very limited understanding is this paper is reporting widely spread damage on the genome. The original bacteria CRISPR can only cut off pre-defined sequences, but the artificial gene tech CRISPR can add and edit as well. I'm wondering if the later two modes are the sources of the genome damage. |
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I was under the impression that addition and editing capability were essentially done by injecting the sequence that is desired and performing the cut. Then you simply rely on the repair mechanisms to have a chance to put the new segment in instead of the old segment. Is that not the case?