[icegreentea]

I wouldn't use 'probably' at all. The level of proof submitted is incredibly light - it only inspects two and a half proteins - and doesn't attempt to inspect the relationship between its proposed mechanism (change in hydration shell density) and protein denaturation.

They show two graphs of protein denaturation curves showing maximum rate change in 50-65 degree range, and a curve of a different protein's hydration shell density changing. At the very least, showing correspondence between hydration shell density and denature curves within a single protein would be significantly more convincing.

While protein folding and interaction is devilishy tricky to compute, the basic idea that injecting extra energy into a system thats held together only by weak hydrogen bonds will disrupt structure and function hardly requires invocation of additional forms of water.

This isn't to say that the claims may not be true. But I would not jump to "probably".

In fact, your quoted statement doesn't even say that proteins become less stable, what the quoted statement says is that a SINGLE protein (lysozyme) undergoes irreversible structural changes over 65 degrees.

We know of a variety of high temperature resistant proteins (Taq Polymerase for example). While is certainly true that most "ordinary" (ie non extremopile) proteins will probably suffer irreversible structural changes in about that temperature change, it's not super great proof.